Determination of high-affinity oestrogen-receptor sites in uterine supernatant preparations
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منابع مشابه
High affinity binding of anti-oestrogen to the chick liver nuclear oestrogen receptor.
Tamoxifen is a potent inhibitor of specific oestrogen-induced yolk protein synthesis by chicken liver. The oestradiol receptor in salt extracts of liver nuclei from oestrogen-treated chicks has a K(d) for oestradiol of 0.7+/-0.2nm. Tamoxifen and its metabolite, monohydroxytamoxifen, compete for binding to the salt-soluble nuclear receptor with K(i) values of 2.6 and 0.1nm respectively. The anti...
متن کاملInteraction of the radiolabelled high-affinity anti-oestrogen [3H]H1285 with the cytoplasmic oestrogen receptor.
The high-affinity triarylethylene anti-oestrogen H1285 [4-(NN-diethylaminoethoxy)-beta-ethyl-alpha-(p-hydroxyphenyl) -4'-methoxystilbene] was tritiated to high specific radioactivity (35 Ci/mmol). Competition experiments between [3H]H1285 and H1285 or oestradiol demonstrated that both compounds would compete with [3H]H1285 for oestrogen-specific binding sites in rat uterine cytosol. [3H]H1285 h...
متن کاملIdentification of high affinity estrogen binding sites in calf uterine microsomal membranes.
Membrane-associated binding sites with high affinity and specificity for estrogens have been identified in calf uterine microsomes. The binding of 17 beta-[3H]estradiol is specific and saturable at low hormone concentration (2 nM) of high affinity (Kd = 0.5 nM) and sensitive to trypsin and other proteolytic enzymes. Binding of [3H]estradiol to membranes is inhibited by low concentrations of unl...
متن کاملQuantitative determination of three angiotensin-II-receptor antagonists in presence of Hydrochlorothiazide by RP-HPLC in their tablets preparations
Losartan potassium, Valsartan , Telmisartan and Irbesartan are angiotensin-II-receptor antagonists (ARA II) group which used in treatment of hypertension alone or in combination with other drugs mainly Hydrochlorothiazide. RP- HPLC method was developed for the assay of three angiotensin-II-receptor antagonists (ARA-IIs) in presence of Hydrochlorothiazide. The method was performed by reversed ph...
متن کاملQuantitative determination of three angiotensin-II-receptor antagonists in presence of Hydrochlorothiazide by RP-HPLC in their tablets preparations
Losartan potassium, Valsartan , Telmisartan and Irbesartan are angiotensin-II-receptor antagonists (ARA II) group which used in treatment of hypertension alone or in combination with other drugs mainly Hydrochlorothiazide. RP- HPLC method was developed for the assay of three angiotensin-II-receptor antagonists (ARA-IIs) in presence of Hydrochlorothiazide. The method was performed by reversed ph...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1200831